Composite

Part:BBa_K5109021:Design

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-27)


Laccase E surface display system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 639
    Illegal BamHI site found at 2245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 900
    Illegal NgoMIV site found at 1121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 667


Design Notes

In order to change the enzyme, with a different one or with a different sequence, the desired sequence must me synthesized with BsaI and BamHI ends.


Source

The sequence was designed on Benchling and then synthesized.

References